Let’s start with what DOT stands for, that is , the Department of Transportation. They are the government agency residing over truck drivers, pilots, heavy equipment operators and other individuals who are deemed to be performing “safety-sensitive” duties. The Department of Transportation is currently the only Federally Regulated entity in terms of drug testing. The DOT requires that 5 specific drugs be included in a DOT test. They are: Marijuana, Cocaine, Amphetamine, Opiates and PCP, often labeled DOT 5-Panel. They also require that strict guidelines be followed in terms of the collection process. Aside from the DOT 5-Panel, we identify another test as the NON-DOT 5-Panel because it is often chosen by facilities who are not mandated to perform DOT testing but want to “mirror” the DOT protocol while including a more heavily abused drug besides PCP. PCP has been on the decline for many years while Methamphetamine use has skyrocketed. This panel tests for Marijuana, Cocaine, Amphetamine, Opiates and Methamphetamine. Typically referred to as the NON-DOT 5-Panel. I hope this sheds some light on the difference between the two.